I have this same problem. Just installed and activated the full version of .NET Developer Bundle, using ANTS Perf Profile 220.127.116.112
I put in my local IIS URL
I set Line Level and Method Level timings
If I leave it as it using "unsued" port doesn't load anything
If I put Orignal Port (IIS will restart) it still doesn't load initialy, I have to reload it myself manually on the browser, hitting enter in the url address bar.
But then it's done and doesn't seem to profile anything, even after selecting ASP.NET and .NET from he Charting options.