Thanks for your forum post. It sounds like one of two major issues: either the Profiler hadn't hooked into your application or the application is not managed code. You may try enabling logging
to see if that pinpoints any problems. You may also want to try the application event logs and look for a "Failed to CoCreate the Profiler" error messages, as this may indicate that the profiler core component has crashed. If not, then the profiler core component may have had trouble setting up a named pipe to connect your process to ANTS Profiler.